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Different Impacts of Short-Chain Fatty Acids on Saturated and Polyunsaturated Fatty Acid Biosynthesis in Aurantiochytrium sp. SD116
journal contribution
posted on 2013-10-16, 00:00 authored by Xiaojin Song, Yanzhen Tan, Yajun Liu, Jingtao Zhang, Guanglei Liu, Yingang Feng, Qiu CuiAurantiochytrium is an important
docosahexaenoic acid (DHA) producer containing two kinds of fatty
acid synthesis pathways, that is, the fatty acid synthase pathway
(FAS) for saturated fatty acid synthesis and the polyketide synthase
pathway (PKS) for polyunsaturated fatty acid synthesis. To understand
the regulation mechanism between the two pathways, the impacts of
six short-chain fatty acids on the fatty acid synthesis of Aurantiochytrium sp. SD116 were studied. All short-chain
fatty acids showed little effect on the cell growth, but some of them
significantly affected lipid accumulation and fatty acid composition.
Pentanoic acid and isovaleric acid greatly inhibited the synthesis
of saturated fatty acids, whereas the polyunsaturated fatty acid synthesis
was not affected. Analysis of malic enzyme activity, which supplied
NADPH for saturated fatty acids biosynthesis, indicated that the two
fatty acid synthesis pathways can utilize different substrates and
possess independent sources of NADPH.