ie8b04386_si_001.pdf (145.04 kB)
Dichlororesorufin-Based Colorimetric and Fluorescent Probe for Ultrasensitive Detection of Mercury Ions in Living Cells and Zebrafish
journal contribution
posted on 2018-12-10, 00:00 authored by Qingxia Duan, Xiaoyu Lv, Caiyun Liu, Zhuofan Geng, Fenfen Zhang, Wenlong Sheng, Zuokai Wang, Pan Jia, Zilu Li, Hanchuang Zhu, Baocun ZhuIn this work, a simple dichlororesorufin-based
colorimetric and
fluorescent probe R-Hg with the recognition receptor
of carbonothioate was designed, synthesized, and exploited to determine
mercury ions in aqueous solution and living cells as well as in zebrafish.
After a series of analytical tests, probe R-Hg showed
some excellent characteristics, such as ultrasensitivity, high selectivity,
and good water solubility. The probe solution exhibited a very large
fluorescence enhancement (about 150-fold) upon addition of Hg2+. Additionally, probe R-Hg exhibited excellent
sensitivity, which could quantificationally detect Hg2+ at the nanomolar level, and the detection limit was 0.49 nM. Moreover,
probe R-Hg could be used as a “naked-eye”
probe for monitoring Hg2+, because the color of its solution
transformed remarkably from yellow into pink with the participation
of Hg2+. Importantly, probe R-Hg has potential
application value and has been successfully applied to the determination
of Hg2+ in practical water samples and in living RAW 264.7
cells as well as in zebrafish as a bioimaging reagent.