ac504868w_si_001.pdf (378.76 kB)
Development and Characterizations of a Miniature Capillary Electrophoresis Mass Spectrometry System
journal contribution
posted on 2015-02-17, 00:00 authored by Muyi He, Zhenhua Xue, Yinna Zhang, Zejian Huang, Xiang Fang, Feng Qu, Zheng Ouyang, Wei XuA miniature capillary electrophoresis
mass spectrometry (CE/MS)
system has been developed in this work. A 100% electrical driven miniaturized
CE device was integrated with a miniature MS instrument, which has
a discontinuous atmospheric pressure interface (DAPI) for coupling
with atmospheric pressure ionization sources. A nanoelectrospray ionization
(nano-ESI) source was developed with a sheath liquid interface for
coupling the miniature CE and the MS system. A systematic characterization
and optimization of the analytical performance have been done. The
analysis of isobaric peptides and avoiding charge competition effects
in nano-ESI sources have been demonstrated.