jf8b03082_si_001.pdf (226.87 kB)
Determination of Five Sex Hormones in Urine Samples for Early Evaluation of Male Reproductive Toxicity Induced by Phthalate Esters in Rats
journal contribution
posted on 2018-09-12, 00:00 authored by Liang-Liang Qian, Rui-Xian Li, Hai-Tao Gao, Chun-Min Wang, Lei Li, Qian Xu, Jian LiIn
this work, male rats were exposed to multiple phthalate esters (MIXPs)
in a long-term low-dose model for the early evaluation of reproductive
toxicity. An ananlysis method with better sensitivity, accuracy and
precision was established to determine the five sex hormones (androstenedione,
testosterone, dehydroepiandrosterone, dihydrotestosterone, and estrone)
in collected urine samples. The results showed that all the analytes
in the MIXPs treated group changed in a time-dependent manner. Specifically,
estrone significantly decreased from the 30th day and the other four
changed from the 30th day and then significantly increased on the
60th day, while no obvious changes were found in the control group.
Therefore, a possible way was provided for the early evaluation of
male reproductive toxicity induced by Phthalate esters (PEs) . The
reliability of judgment was improved by observing the changes of five
target hormones simultaneously. Furthermore, good compliance was predicted
for the practical application due to the noninvasive and convenient
urine sample collection.