pr9b00662_si_001.pdf (764.25 kB)
Detergent-Free Simultaneous Sample Preparation Method for Proteomics and Metabolomics
journal contribution
posted on 2019-12-12, 17:36 authored by Alexandre Zougman, John P. Wilson, Lee D. Roberts, Rosamonde E. BanksThe integration of omics techniques has seen a step change
in our
understanding of biological systems. However, multiomics has been
impaired by mutually exclusive omic separation methods and the destructive
nature of the techniques when sample is limited. We describe Simultaneous
Trapping (SiTrap), a simple and effective detergent-free method that
facilitates direct measurement of the proteome and metabolome in the
same sample extract. This “single-pot” multiomics processing
is particularly beneficial in cases when sample amounts are limited
or are heterogeneous, for example, tissue biopsies. We demonstrate
the value of the SiTrap methodology as an essential multiomics tool
in a proof-of-principle integrated study of renal cancer tissue biopsy
samples. We believe SiTrap has the potential to become an indispensable
tool in translational medical research.