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Designing a Tenascin-C-Inspired Short Bioactive Peptide Scaffold to Direct and Control Cellular Behavior
journal contribution
posted on 2019-11-21, 16:36 authored by Pooja Sharma, Harsimran Kaur, Sangita RoyOver the last decade, synthetically designed scaffolds
are emerging
as promising biomaterials for tissue engineering applications. In
this context, peptide hydrogels are gaining wide attention, owing
to their ability to mimic structural and functional complexity of
the natural extracellular matrix (ECM). To this end, exploration of
minimalistic bioactive peptide sequences for the fabrication of tissue
engineering scaffolds provides a competitive edge over the conventional
design principles where complex sequences were mainly explored for
scaffold formation. In the present study, we explored the self-assembling
potential of minimalistic bioactive peptide sequence from tenascin-C
protein and its application in supporting cellular viability and proliferation.
Tenascin-C is a multimeric protein known to express in adult tissues
mainly during tissue injury or remodeling. To the best of our knowledge,
the designed octapeptide is the shortest bioactive sequence derived
solely from tenascin-C, which is known in the literature to impart
specific bioactivity. The short peptide sequence showed high propensity
to form a nanofibrous network at physiological pH, which was further
entangled to form a macroscopic hydrogel network. Interestingly, the
gels were found to be mechanoresponsive and thixotropic which opens
up the scope for utilizing them as designer injectable matrices. These
novel hydrogels supported the growth and proliferation of cells of
both neural and non-neural ECM origin. However, neural cells cultured
on these bioactive hydrogels showed normal β-III tubulin expression,
highlighting their specific potential to be further explored for tissue
engineering applications.
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minimalistic bioactive peptide sequenceBioactive Peptide Scaffoldadult tissuesβ- III tubulin expressionpeptide sequencetissue engineering applicationsproliferationmacroscopic hydrogel networkminimalistic bioactive peptide sequencesdesigner injectable matricesnon-neural ECM originpeptide hydrogelstissue injuryscaffold formationControl Cellular Behaviortenascin-C proteinbioactive sequencebioactive hydrogelsextracellular matrixmultimeric proteinnovel hydrogelsdesign principlestissue engineering scaffoldsnanofibrous network
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