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Design of Thermoresponsive Polymers Toward Antibody Purification
journal contribution
posted on 2019-05-31, 00:00 authored by Kana Nishimori, Masashi Maruyama, Yuzuru Shimazaki, Makoto Ouchi, Hiroshi YoshidaWe
proposed a concept of a poly(N-isopropylacrylamide)
(PNIPAM)-based thermoresponsive antibody adsorbing material toward
antibody purification; the end-functionalized PNIPAM chain can afford
to adsorb antibodies below LCST and desorb upon heating due to the
thermal response feature. To approach the concept, we synthesized
telechelic PNIPAM derivatives through atom transfer radical polymerization
(ATRP) and post reactions; an immunoglobulin G (IgG) binding ligand
was incorporated at the α-terminal and some carboxylic acid
groups for immobilization were embedded at another terminal. The thermoresponsive
behaviors were characterized by a temperature-variable turbidity measurement
and surface plasmon resonance (SPR) measurement.
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surface plasmon resonanceconceptresponse featureα- terminalAntibody Purificationimmunoglobulin GSPRtemperature-variable turbidity measurementthermoresponsive behaviorsbinding ligandATRPantibody purificationatom transfertelechelic PNIPAM derivativesLCSTend-functionalized PNIPAM chaincarboxylic acid groupsThermoresponsive Polymersadsorb antibodiespost reactions
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