Design of Thermoresponsive Polymers Toward Antibody Purification

We proposed a concept of a poly­(N-isopropylacrylamide) (PNIPAM)-based thermoresponsive antibody adsorbing material toward antibody purification; the end-functionalized PNIPAM chain can afford to adsorb antibodies below LCST and desorb upon heating due to the thermal response feature. To approach the concept, we synthesized telechelic PNIPAM derivatives through atom transfer radical polymerization (ATRP) and post reactions; an immunoglobulin G (IgG) binding ligand was incorporated at the α-terminal and some carboxylic acid groups for immobilization were embedded at another terminal. The thermoresponsive behaviors were characterized by a temperature-variable turbidity measurement and surface plasmon resonance (SPR) measurement.