ac9b02605_si_001.pdf (1.22 MB)
Covalent Functionalization of Bovine Serum Albumin with Graphene Quantum Dots for Stereospecific Molecular Recognition
journal contribution
posted on 2019-08-23, 11:33 authored by Qiumin Ye, Lili Guo, Datong Wu, Baozhu Yang, Yongxin Tao, Linhong Deng, Yong KongStereospecific molecular
recognition with simple and easily available
proteins is of significant importance in life science and biomaterial
science. Herein, we report on a chiral sensing platform, graphene
quantum dots (GQDs)-functionalized bovine serum albumin (BSA), for
chiral recognition of tryptophan (Trp) isomers. Amidation reaction
between BSA and GQDs was directly responsible for the introduction
of GQDs to BSA, resulting in significant changes in the spatial configuration
of BSA and the exposure of more chiral sites at the protein surface.
The BSA–GQDs-based chiral sensor exhibited good biomolecular
homochirality in the recognition of Trp isomers, and the higher affinity
of BSA–GQDs toward l-Trp than its isomer, d-Trp, was also revealed by density functional theory (DFT) considering
the possible hydrogen bonds between the Trp isomers and the solvent-accessible
residues of BSA.