mz5001465_si_001.pdf (1.97 MB)
Convenient Preparation of Bactericidal Hydrogels by Covalent Attachment of Stabilized Antimicrobial Peptides Using Thiol–ene Click Chemistry
journal contribution
posted on 2014-05-20, 00:00 authored by Rik T.
C. Cleophas, Martijn Riool, H. (Linda) C. Quarles van Ufford, Sebastian A. J. Zaat, John A. W. Kruijtzer, Rob M. J. LiskampThis report describes the design
and synthesis of a bactericidal
poly(ethylene glycol)-based (PEG) hydrogel coating with covalently
attached antimicrobial peptides (AMP) stabilized against proteolytic
degradation. As such, mimics of the highly active AMP HHC10 (H-KRWWKWIRW-NH2) were designed for optimal stability in human serum while
retaining strong antimicrobial activity against Staphylococcus
aureus and Staphylococcus epidermidis, the major causative agents of biomaterial associated infection.
In order to investigate the selectivity of the AMPs, their hemolytic
activity was determined. A N-terminal cysteine facilitated thiol–ene
chemistry for a fast, single-step immobilization/photopolymerization
strategy. The antimicrobial activity of the resulting thin layer hydrogel
coating on a PET surface was established using the Japanese Industrial
Standard (JIS) Z2801 assay, showing complete killing (>99.9%) of
inocula
of S. aureus ATCC 49230, S. epidermidis ATCC 35984, and E.
coli ATCC 8739.