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Controlling the Pore Structure of Collagen Sponge by Adjusting the Cross-Linking Degree for Construction of Heterogeneous Double-Layer Bone Barrier Membranes
journal contribution
posted on 2020-03-18, 14:45 authored by Xi Zhao, Xinying Li, Xiaofen Xie, Jinfeng Lei, Liming Ge, Lun Yuan, Defu Li, Changdao MuGuided
bone regeneration (GBR) has been regarded as a valuable
way to effectively induce bone remodeling. The key factor of GBR is
to place a barrier membrane between the soft tissue and bone defect,
preventing the untimely intrusion of fibroblasts and permitting the
prior settlement of internal osteoblasts. Notably, heterogeneous double-layer
GBR membranes with a compact upper layer and a loose lower layer exhibit
enhanced effectiveness in blocking fibroblasts and promoting the growth
of osteoblasts. Herein, we present porous and interconnected collagen-based
sponges with controllable pore size for the fabrication of absorbable
GBR membranes with a heterogeneous double-layer structure. Dialdehyde
carboxymethyl cellulose was used to fix collagen-based sponges. The
pore size of the sponges can be well controlled by adjusting the cross-linking
degree, which is decreased with an increase of cross-linking degree.
The sponges show enhanced mechanical properties, inhibited swelling
ability and biodegradation, good blood compatibility, and good cytocompatibility.
The sponges are feasible to form a heterogeneous double-layer structure
with a loose lower layer and a compact upper layer. Interestingly,
the lower layer with the pore size of 200–300 μm can
promote the adhesion, proliferation, and differentiation of osteoblasts
MC3T3-E1 cells, while the upper layer with the pore size of 20–50
μm makes a great contribution to the growth of myoblast C2C12
cells. Overall, the collagen-based sponges have the potential to be
used to fabricate heterogeneous double-layer bone barrier membranes
for bone remodeling.
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collagen-based spongesabsorbable GBR membranesosteoblasts MC 3T cellsmyoblast C 2C cellsdouble-layer GBR membranesdouble-layer structurepore sizeDialdehyde carboxymethyl celluloseHeterogeneous Double-Layer Bone Barrier Membranes Guided bone regenerationcross-linking degreedouble-layer bone barrier membranes
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