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Controlling the Formation of Phospholipid Monolayer, Bilayer, and Intact Vesicle Layer on Graphene
journal contribution
posted on 2016-04-19, 00:00 authored by Seyed R. Tabaei, Wei Beng Ng, Sang-Joon Cho, Nam-Joon ChoExciting
progress has been made in the use of graphene for bio- and chemical
sensing applications. In this regard, interfacing lipid membranes
with graphene provides a high-sealing interface that is resistant
to nonspecific protein adsorption and suitable for measuring biomembrane-associated
interactions. However, a controllable method to form well-defined
lipid bilayer coatings remains elusive, and there are varying results
in the literature. Herein, we demonstrate how design strategies based
on molecular self-assembly and surface chemistry can be employed to
coat graphene surface with different classes of lipid membrane architectures.
We characterize the self-assembly of lipid membranes on CVD-graphene
using quartz crystal microbalance with dissipation, field-effect transistor,
and Raman spectroscopy. By employing the solvent-assisted lipid bilayer
(SALB) method, a lipid monolayer and bilayer were formed on pristine
and oxygen-plasma-treated CVD-graphene, respectively. On these surfaces,
vesicle fusion method resulted in formation of a lipid monolayer and
intact vesicle layer, respectively. Collectively, these findings provide
the basis for improved surface functionalization strategies on graphene
toward bioelectronic applications.