posted on 2020-03-23, 21:31authored byTaehong Kwon, Sung Hee Ko, Jean-François P. Hamel, Jongyoon Han
We
demonstrate a new micro/nanofluidic system for continuous and
automatic monitoring of protein product size and quantity directly
from the culture supernatant during a high-cell-concentration CHO
cell perfusion culture. A microfluidic device enables clog-free cell
retention for a bench-scale (350 mL) perfusion bioreactor that continuously
produces the culture supernatant containing monoclonal antibodies
(IgG1). A nanofluidic device directly monitors the protein
size and quantity in the culture supernatant. The continuous-flow
and fully automated operation of this nanofluidic protein analytics
reduces design complexity and offers more detailed information on
protein products than offline and batch-mode conventional analytics.
Moreover, chemical and mechanical robustness of the nanofluidic device
enables continuous monitoring for several days to a week. This continuous
and online protein quality monitoring could be deployed at different
steps and scales of biomanufacturing to improve product quality and
manufacturing efficiency.