ab0c00321_si_001.pdf (2.93 MB)
Continuous Formation of Ultrathin, Strong Collagen Sheets with Tunable Anisotropy and Compaction
journal contribution
posted on 2020-06-19, 12:03 authored by Shashi Malladi, David Miranda-Nieves, Lian Leng, Stephanie J. Grainger, Constantine Tarabanis, Alexander P. Nesmith, Revanth Kosaraju, Carolyn A. Haller, Kevin Kit Parker, Elliot L. Chaikof, Axel GüntherThe multiscale organization
of protein-based fibrillar materials
is a hallmark of many organs, but the recapitulation of hierarchal
structures down to fibrillar scales, which is a requirement for withstanding
physiological loading forces, has been challenging. We present a microfluidic
strategy for the continuous, large-scale formation of strong, handleable,
free-standing, multicentimeter-wide collagen sheets of unprecedented
thinness through the application of hydrodynamic focusing with the
simultaneous imposition of strain. Sheets as thin as 1.9 μm
displayed tensile strengths of 0.5–2.7 MPa, Young’s
moduli of 3–36 MPa, and modulated the diffusion of molecules
as a function of collagen nanoscale structure. Smooth muscle cells
cultured on engineered sheets oriented in the direction of aligned
collagen fibrils and generated coordinated vasomotor responses. The
described biofabrication approach enables rapid formation of ultrathin
collagen sheets that withstand physiologically relevant loads for
applications in tissue engineering and regenerative medicine, as well
as in organ-on-chip and biohybrid devices.
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Keywords
biofabrication approachTunable Anisotropy1.9 μ mMPacollagen fibrilsformationloading forcesmulticentimeter-wide collagen sheetsbiohybrid devicestissue engineeringregenerative medicineSmooth muscle cellsfibrillar scalesvasomotor responsesContinuous Formationmicrofluidic strategyStrong Collagen Sheetsultrathin collagen sheetsprotein-based fibrillar materialshierarchal structuresapplicationmultiscale organizationcollagen nanoscale structure
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