ja407392n_si_001.pdf (776.73 kB)
Complexin‑1 Enhances the On-Rate of Vesicle Docking via Simultaneous SNARE and Membrane Interactions
journal contribution
posted on 2015-12-16, 23:41 authored by Jiajie Diao, Daniel
J. Cipriano, Minglei Zhao, Yunxiang Zhang, Sachi Shah, Mark S. Padolina, Richard
A. Pfuetzner, Axel T. BrungerIn
synaptic terminals, complexin is thought to have inhibitory
and activating roles for spontaneous “mini” release
and evoked synchronized neurotransmitter release, respectively. We
used single vesicle–vesicle microscopy imaging to study the
effect of complexin-1 on the on-rate of docking between vesicles that
mimic synaptic vesicles and the plasma membrane. We found that complexin-1
enhances the on-rate of docking of synaptic vesicle mimics containing
full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasma
membrane-mimicking vesicles containing full-length syntaxin-1A and
SNAP-25A. This effect requires the C-terminal domain of complexin-1,
which binds to the membrane, the presence of PS in the membrane, and
the core region of complexin-1, which binds to the SNARE complex.