pr501167u_si_001.pdf (2.77 MB)
Comparison of Two Different Astragali Radix by a 1H NMR-Based Metabolomic Approach
journal contribution
posted on 2015-05-01, 00:00 authored by Ai-Ping Li, Zhen-Yu Li, Hai-Feng Sun, Ke Li, Xue-Mei Qin, Guan-Hua DuAstragali Radix (AR) is a commonly
used herbal drug in traditional
chinese medicine and is widely used for the treatment of diabetes,
cardiovascular diseases, nephropathy, and neuropathy. The main source
of AR in China is the dried root of Astragalus
membranaceus var. mongholicus (Bge.) Hsiao, and both cultivated and wild ARs are used clinically.
A systematic comparison of cultivated AR (GS-AR) and wild AR (SX-AR)
should be performed to ensure
the clinical efficacy and safety. In this study, the chemical composition
of the two different ARs, which were collected in the Shanxi (wild)
and Gansu (cultivated) provinces, were compared by NMR-based metabolic
fingerprint coupled with multivariate analysis. The SX-AR- and GS-AR-induced
metabolic changes in the endogenous metabolites in mice were also
compared. The results showed that SX-AR and GS-AR differed significantly
not only in the primary metabolites but also in the secondary metabolites.
However, alterations among the endogenous metabolites in the serum,
lung, liver, and spleen were relatively small. This study provided
a novel and valuable method for the evaluation of the consistency
and diversity of herbal drugs, and further studies should be conducted
on the difference in polysaccharides as well as the biological effects
between the two kinds of AR.