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Comparison of Regenerative Effects of Transplanting Three-Dimensional Longitudinal Scaffold Loaded-Human Mesenchymal Stem Cells and Human Neural Stem Cells on Spinal Cord Completely Transected Rats
journal contribution
posted on 2020-02-19, 20:49 authored by Yunlong Zou, Yannan Zhao, Zhifeng Xiao, Bing Chen, Dezun Ma, He Shen, Rui Gu, Jianwu DaiStem
cell-based therapy has been considered as a potential treatment
to restore spinal cord injury (SCI) through reconstructing neural
networks and providing a favorable microenvironment for neuronal survival,
differentiation, and axonal outgrowth. Biomaterial scaffolds can promote
cell attachment and survival, neuronal differentiation, and axonal
outgrowth; therefore, they were used to combine with stem cells for
implantation in SCI treatment. In addition, a longitudinal scaffold
can guide regenerated axons with orientated growth and axial extension.
Both human umbilical cord-derived mesenchymal stem cells (hMSCs) and
human fetal spinal cord-derived neural stem cells (hNSCs) have been
applied in clinical trials worldwide. To our knowledge, a parallel
comparison of the therapeutic effects of hMSC and hNSC implantations
has not been conducted. Hence, in this study, we grafted hMSCs or
hNSCs seeded on longitudinal collagen sponge scaffolds into rats with
completely transected SCI to examine differences in SCI repair. Both
hMSCs and hNSCs had equivalent effects on reducing glial scar formation
around the lesion gap. More neuronal class III β-tubulin-positive
neurons and neurofilament-positive nerve fibers were found in the
lesion cavity after hNSC implantation. In addition, hNSCs had better
capabilities to improve motor function, attenuate inflammation, and
promote cell survival than hMSCs. These encouraging results provide
a clinical basis for future stem cell-based SCI therapies.
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Transplanting Three-Dimensional Longitudinal Scaffold Loaded-Human Mesenchymal Stem CellsSpinal Cord Completely Transected Rats Stem cell-based therapyhMSCsurvivalglial scar formationHuman Neural Stem Cellscell-based SCI therapiesclass III β- tubulin-positive neuronsaxonal outgrowthimplantationhNSCneurofilament-positive nerve fiberscollagen sponge scaffoldsumbilical cord-derived mesenchymal
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