bm9b01204_si_001.pdf (388.83 kB)
Comparison of Photo Cross Linkable Gelatin Derivatives and Initiators for Three-Dimensional Extrusion Bioprinting
journal contribution
posted on 2019-12-09, 18:45 authored by Thomas
J. Tigner, Satyam Rajput, Akhilesh K. Gaharwar, Daniel L. AlgeThe objective of this study was to evaluate the utility
of gelatin–norbornene
(GelNB), which is cross-linkable via thiol–ene click chemistry,
and the photoinitiator lithium phenyl-2,4,6 trimethylbenzoylphosphinate
(LAP) for 3D bioprinting. These materials were compared to two widely
used materials, gelatin-methacryloyl (GelMA) and 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone
(I2959). Characterization of photocuring kinetics revealed that LAP
markedly improved the kinetics compared to I2959, which improved stability
and print fidelity. Additionally, GelNB exhibited improved photocuring
kinetics, improved stability, and decreased filament spreading compared
to GelMA. However, inks containing GelMA yielded at lower stress,
were more easily extruded, and produced smoother filaments. NIH 3T3
fibroblasts exhibited high viability in printed constructs, regardless
of the gelatin derivative or photoinitiator used. Overall, these results
support the selection of LAP over I2959 and suggest that GelNB could
be a useful alternative to GelMA, although further work is needed
to optimize GelNB extrusion.