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Combining Time-of-Flight Secondary Ion Mass Spectrometry Imaging Mass Spectrometry and CARS Microspectroscopy Reveals Lipid Patterns Reminiscent of Gene Expression Patterns in the Wing Imaginal Disc of Drosophila melanogaster
journal contribution
posted on 2017-07-20, 00:00 authored by Florian Marty, Gianluca Rago, Donald F. Smith, Xiaoli Gao, Gert B. Eijkel, Luke MacAleese, Mischa Bonn, Erich Brunner, Konrad Basler, Ron M. A. HeerenUsing
label-free ToF-SIMS imaging mass spectrometry, we generated
a map of small molecules differentially expressed in the Drosophila wing imaginal disc. The distributions of these moieties were in
line with gene expression patterns observed during wing imaginal disc
development. Combining ToF-SIMS imaging and coherent anti-Stokes Raman
spectroscopy (CARS) microspectroscopy allowed us to locally identify
acylglycerols as the main constituents of the pattern differentiating
the future body wall tissue from the wing blade tissue. The findings
presented herein clearly demonstrate that lipid localization patterns
are strongly correlated with a developmental gene expression. From
this correlation, we hypothesize that lipids play a so far unrecognized
role in organ development.
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lipid localization patternsGene Expression Patternsorgan developmentmolecules differentiallyDrosophila wing imaginal discWing Imaginal DiscDrosophila melanogasterIon Mass Spectrometry Imaging Mass SpectrometryToF-SIMS imaginganti-Stokes Raman spectroscopygene expression patternslabel-free ToF-SIMS imaging mass spectrometrywing imaginal disc developmentfuture body wall tissuewing blade tissueLipid Patterns ReminiscentCARS Microspectroscopygene expression
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