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Coexpression of Methyltransferase Gene dmt50 and Methylene Tetrahydrofolate Reductase Gene Increases Arabidopsis thaliana Dicamba Resistance
journal contribution
posted on 2019-01-17, 00:00 authored by Le Chen, Shigang Yao, Tao Chen, Qin Tao, Xiangting Xie, Xiang Xiao, Derong Ding, Qin He, Jian HeDicamba,
a broad-spectrum and highly efficient herbicide, is an
excellent target herbicide for the engineering of herbicide-resistant
crops. In this study, a new tetrahydrofolate (THF)-dependent dicamba
methyltransferase gene, dmt50, was cloned from the
dicamba-degrading strain Rhizorhabdus dicambivorans Ndbn-20. Dmt50 catalyzed the methyl transfer from dicamba to THF,
generating the herbicidally inactive product 3,6-dichlorosalicylic
acid (3,6-DCSA) and 5-methyl-THF. A dmt50 transgenic Arabidopsis thaliana clearly showed dicamba resistance (560
g/ha, the normal field application rate). However, Dmt50 demethylation
activity was inhibited by the product 5-methyl-THF. Mthfr66, encoded
by the 5,10-methylene-THF reductase gene mthfr66 could
relieve the inhibition by removing 5-methyl-THF in vitro. Compared
with expression of dmt50 alone, simultaneous expression
of dmt50 and mthfr66 further improved
the dicamba resistance (1120 g/ha) of transgenic A. thaliana. This study provides new genes for dicamba detoxification and a
strategy for the engineering of dicamba-resistant crops.
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resistanceproduct 5- methyl-THF5- methyl-THFherbicidedicamba-degrading strain Rhizorhabdus dicambivorans Ndbn -20. Dmt 50mthfrDCSAMethyltransferase Gene dmt 50Dmt 50 demethylation activitydmt 50geneexpressionfield application rateMethylene Tetrahydrofolate Reductase Gene Increases Arabidopsis thaliana Dicamba Resistance Dicamba
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