nl0c00295_si_001.pdf (2.06 MB)
Cobalt-Directed Assembly of Antibodies onto Metal–Phenolic Networks for Enhanced Particle Targeting
journal contribution
posted on 2020-03-10, 16:53 authored by Wenjie Zhang, Quinn A. Besford, Andrew J. Christofferson, Patrick Charchar, Joseph J. Richardson, Aaron Elbourne, Kristian Kempe, Christoph E. Hagemeyer, Matthew R. Field, Chris F. McConville, Irene Yarovsky, Frank CarusoThe
orientation-specific immobilization of antibodies onto nanoparticles,
to preserve antibody–antigen recognition, is a key challenge
in developing targeted nanomedicines. Herein, we report the targeting
ability of metal–phenolic network (MPN)-coated gold nanoparticles
with surface-physisorbed antibodies against respective antigens. The
MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies,
all particle systems exhibited enhanced association with target antigens,
with CoII systems demonstrating more than 2-fold greater
association. These systems contained more metal atoms distributed
in a way to specifically interact with antibodies, which were investigated
by molecular dynamics simulations. A model antibody fragment crystallizable
(Fc) region in solution with CoII-tannic acid complexes
revealed that the solvent-exposed CoII can directly coordinate
to the histidine-rich portion of the Fc region. This one-pot interaction
suggests anchoring of the antibody Fc region to the MPN on nanoparticles,
allowing for enhanced targeting.