ac0c00227_si_001.pdf (1.39 MB)
Co-Incorporated Mesoporous Carbon Material-Assisted Laser Desorption/Ionization Ion Source as an Online Interface of In Vivo Microdialysis Coupled with Mass Spectrometry
journal contribution
posted on 2020-03-24, 14:38 authored by Na Li, Shumu Li, Tuo Li, Hui Yang, Yangyang Zhang, Zhenwen ZhaoThe
combination of microdialysis and mass spectrometry (MS) provides
the potential for rapidly monitoring diverse metabolites in vivo.
Unfortunately, the high concentration of salt in biological microdialysates
hindered the sensitive and online detection of these small molecular
compounds. In this study, we synthesized Co-incorporated mesoporous
carbon material (Co-NC) and developed a Co-NC-assisted laser desorption/ionization
(LDI) ion source as an online interface of in vivo microdialysis coupled
with MS for the direct analysis of diverse metabolites in microdialysates.
The Co-NC could be used as a matrix for surface-assisted laser desorption/ionization
mass spectrometry (SALDI MS) analysis of small molecular compounds,
even under high concentration salt conditions. The Co-NC possessed
the adsorption ability for small molecular compounds, and it was believed
that the adsorption ability of Co-NC might separate the analytes from
the salt in microdialysates at a microscopic level, which might facilitate
the desorption and ionization of the analytes and finally improved
the salt-tolerance ability as a matrix. Furthermore, the Co-NC-assisted
LDI ion source as a novel interface of in vivo microdialysis coupled
with MS has been applied to the online monitoring of liver metabolites
from the CCl4-induced liver injury rat model for the first
time.