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Cleancap-Regulated Aggregation-Induced Emission Strategy for Highly Specific Analysis of Enzyme
journal contribution
posted on 2020-03-18, 14:33 authored by Yuanyuan Huang, Longyi Zhu, Jiahao Ji, Yang Li, Tianrui Liu, Jianping LeiIn this work, a cleancap-regulated
aggregation-induced emission
(AIE) strategy based on copper nanoclusters (CuNCs) was developed
with stepwise recognition for highly specific analysis of the enzyme.
The dissolved CuNCs with AIE characteristics in alkaline solution
were prepared by using p-mercaptophenylboronic acid
as the reducing agent and the stabilizing ligand. The prepared CuNCs
can specifically conjugate with glucose (Glu) to connect with each
other via the rapid boronate esters formation between boronic acids
of CuNCs and a pair of cis-diols on Glu. The cleancap-regulated
AIE strategy was further identified by modification of CuNCs with d-glucose 6-phosphate (P-Glu) as the capper and substrate. Introduction
of alkaline phosphatase to the P-Glu/CuNCs complex can induce the
cleavage of phosphate group to activate the 5,6-diol of Glu on the
CuNCs. The decapped complexes could be aggregated through further
conjugation between 5,6-diol and boronic acid of two CuNCs, resulting
in strong red AIE luminescence. The dual recognitions of enzymatic
cleavage and cis-diols/boronic acid conjugation endow
the designed method with highly specific detection and cell imaging
of enzymatic activity. The cleancap-regulated AIE strategy provides
a universal tool for regulation of AIE phenomenon in trace analysis.
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Keywords
cell imagingdecapped complexesCleancap-Regulated Aggregation-Induced Emission Strategycleancap-regulated AIE strategyboronic acidd-glucose 6- phosphatediolboronic acidsGlumercaptophenylboronic acidtrace analysisAIE phenomenonCuNCboronate esters formationcleancap-regulated aggregation-induced emissioncopper nanoclustersphosphate groupAIE luminescenceAIE characteristicsSpecific Analysis
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