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Chimeric Aptamers-Based and MoS2 Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy for Adenosine Triphosphate Detection
journal contribution
posted on 2018-10-23, 00:00 authored by Yao-Yao Fan, Zhao-Li Mou, Man Wang, Jun Li, Jing Zhang, Fu-Quan Dang, Zhi-Qi ZhangAdenosine
triphosphate (ATP) as a primary energy source plays a
unique role in the regulation of all cellular events. The necessity
to detect ATP requires sensitive and accurate quantitative analytical
strategies. Herein, we present our study of developing a MoS2 nanosheet-enhanced aptasensor for fluorescence polarization-based
ATP detection. A bifunctional DNA strand was designed to consist of
chimeric aptamers that recognize and capture ATP and berberine, a
fluorescence enhancer. In the absence of ATP, the DNA strand bound
to berberine will be hydrolyzed when Exonuclease I (Exo I) is introduced,
releasing berberine as a result. In contrast, when ATP is present,
ATP aptamer folds into a G-quadruplex structure; thus, the complex
can resist degradation by Exo I to maintain berberine for fluorescent
detection purpose. In addition, to magnify the fluorescence polarization
(FP) signal, MoS2 nanosheets were also adopted in the system.
This nanosheets-enhanced FP strategy is simple and facile which does
not require traditional dye-labeled DNA strands and complex operation
steps. The developed fluorescence polarization aptasensor showed high
sensitivity for the quantification of ATP with a detection limit of
34.4 nM, performing well both in buffer solution and in biological
samples.
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MoS 2 nanosheetsATP aptamer foldsberberine34.4 nMDNA strandoperation stepsfluorescence polarization-based ATP detectionbifunctional DNA strandfluorescence polarizationG-quadruplex structuredetection purposefluorescence polarization aptasensornanosheets-enhanced FP strategyMoS 2 nanosheet-enhanced aptasensorchimeric aptamersfluorescence enhancerChimeric Aptamers-Basedenergy sourceAdenosine Triphosphate Detection Adenosine triphosphatedetection limitbuffer solutiondye-labeled DNA strandsMoS 2 Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy
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