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Chemical Characterization of Novel Natural Products from the Roots of Asian Rice (Oryza sativa) that Control Adipocyte and Osteoblast Differentiation
journal contribution
posted on 2018-02-14, 00:00 authored by Hee Rae Kang, Hyung Sik Yun, Tae Kyoung Lee, Seulah Lee, Seon-Hee Kim, Eunjung Moon, Ki-Moon Park, Ki Hyun KimOryza sativa L. is consumed globally as a staple
food, and its roots have been used as a Korean and Chinese medical
supplement for protection of the stomach and lungs and for amelioration
of vomiting and fever. In our continuing search for biologically effective
metabolites from Korean natural materials, we found that an EtOH extract
of O. sativa root reciprocally regulated adipocyte
and osteoblast differentiation. Chemical analysis of the EtOH extract
using a bioassay-guided fractionation protocol led to the isolation
and determination of two novel lignans, oryzativols A and B, responsible
for these regulatory activities. Using 1D and 2D nuclear magnetic
resonance spectroscopic analyses, high-resolution mass spectrometry,
and circular dichroism analysis, the structures of the novel compounds
were elucidated. We examined their effects on the regulation of mesenchymal
stem cell differentiation. Treatment with oryzativol A in the human
mesenchymal cell line C3H10T1/2 suppressed gene expression of peroxisome
proliferator activated receptor γ, which resulted in a reduction
in adipogenesis. Oryzativol A also enhanced the expression of Runx2
and cellular differentiation into osteoblasts in the same mesenchymal
stem cell line.
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resonance spectroscopic analyseschemical analysissativa root reciprocally2 Ddichroism analysismesenchymal cell line C 3Hnovel lignanscell linemass spectrometryAsian Ricegene expressionnovel compoundsperoxisome proliferatorNovel Natural ProductsEtOH1 DRunx 2chemical Characterizationosteoblast differentiationOsteoblast Differentiation Oryza sativa Lbioassay-guided fractionation protocoloryzativolstaple foodcell differentiationOryza sativaControl Adipocyte
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