la7b03403_si_001.pdf (8.45 MB)
Characterization of Robust and Free-Standing 2D-Nanomembranes of UV-Polymerized Diacetylene Lipids
journal contribution
posted on 2018-02-27, 00:00 authored by Roland Hillmann, Martina Viefhues, Lukas Goett-Zink, Dominic Gilzer, Thomas Hellweg, Armin Gölzhäuser, Tilman Kottke, Dario AnselmettiFree-standing
lipid membranes are promising as artificial functional
membrane systems for application in separation, filtration, and nanopore
sensing. To improve the mechanical properties of lipid membranes,
UV-polymerized lipids have been introduced. We investigated free-standing
as well as substrate-supported monolayers of 1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (PTPE) and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) and characterized
them with respect to their structure, morphology, and stability. Using
helium ion microscopy (HIM), we were able to visualize the integrity
of the lipid 2D-nanomembranes spanning micrometer-sized voids under
high-vacuum conditions. Atomic force microscopy (AFM) investigations
under ambient conditions revealed formation of intact and robust pore-spanning
2D-nanomembranes up to 8 × 2 μm2 in size. Analysis
by attenuated total reflection–Fourier transform infrared spectroscopy
(ATR-FTIR) verified a distinct reduction of signal at 2143 cm–1 from diacetylene groups in the 2D-nanomembranes after
UV-polymerization. Further high-resolution AFM investigations of unpolymerized
lipid monolayers revealed a well-ordered two-dimensional network,
when deposited on highly oriented pyrolytic graphite (HOPG). These
structures were inhibited for polymerized adlayers. Structural models
for the molecular arrangement of the adlayers are proposed and discussed.
History
Usage metrics
Categories
Keywords
diacetylene groupsglycero -3-phosphocholine2 D-nanomembranesHIMFree-Standing 2 D-NanomembranesAFM investigationsUV-Polymerized Diacetylene Lipids Free-standing lipid membranesunpolymerized lipid monolayerslipid membraneshigh-vacuum conditionsHOPGlipid 2 D-nanomembranespyrolytic graphiteATR-FTIRAtomic force microscopyhelium ion microscopypore-spanning 2 D-nanomembranesUV-polymerized lipidsglycero -3-phosphoethanolaminePTPEStructural modelsmembrane systemspolymerized adlayerssubstrate-supported monolayerssnambient conditions
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC