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Characterization of Quinoline Yellow Dyes As Transient Aryl Hydrocarbon Receptor Agonists
journal contribution
posted on 2020-02-10, 15:07 authored by Patrick Tarnow, Catrin Zordick, Alex Bottke, Berit Fischer, Friederike Kühne, Tewes Tralau, Andreas LuchThe aryl hydrocarbon
receptor (AHR) and estrogen receptor alpha
(ERα) are two ligand activated transcription factors that are
targeted by a wide range of anthropogenic compounds. Crosstalk between
both receptors is well established but little understood. We previously
developed a dual color luciferase assay (i.e., XEER) which allows
time dissolved monitoring of the activation of both receptors in situ.
The system was now used in conjunction with HPLC-qTOF to identify
several quinophthalone dyes as transient receptor agonists of the
AHR. Altogether the approach identified three widely used dyes, that
is the plastic colorant latyl yellow 3G (LY), the structurally related
textile dye disperse yellow 64 (DY), and the cosmetic dye quinoline
yellow (QY). The latter was the most potent agonist followed by LY
and DY as confirmed by the XEER assay and CYP1A1 gene
induction in MCF7 cells. In addition QY, LY, and DY also inhibited
ER signaling in an AHR-dependent manner. This establishes some evidence
for quinoline yellow dyes as potential disruptors of AHR/ER signaling,
raising potential toxicological concern. Although none of the dyes
featured any signs of genotoxicity in vitro, our data point to the
need for a systematic approach when screening for substances of potential
toxicological and endocrine relevance.
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Quinoline Yellow Dyesaryl hydrocarbon receptoranthropogenic compoundstextile dyeestrogen receptoraddition QYAHR-dependent mannerdata pointplastic colorant latylCYP 1A gene inductionMCF 7 cellstranscription factorsquinophthalone dyesXEER assaytoxicological concern3 Gcolor luciferase assaydye quinolineDYTransient Aryl Hydrocarbon Receptor AgonistsLYreceptor agonists
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