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Cervical Cancer HeLa Cell Autocrine Apoptosis Induced by Coimmobilized IFN-γ plus TNF-α Biomaterials
journal contribution
posted on 2018-02-13, 00:00 authored by Jian Li, Yuxiao Zhang, Liyi Chen, Xinhua Lu, Zhibin Li, Yongyong Xue, Yan-Qing GuanUsing
external methods to induce the death of cancer cells is recognized
as one of the main strategies for cancer treatment. Research indicated
that TNF-α is frequently used in tumor biotherapy while IFN-γ
can directly inhibit tumor cell proliferation. In our study, TNF-α
and IFN-γ were coimmobilized on polystyrene material (PSt) or
Fe3O4–oleic acid nanoparticles (NPs).
Then the structural change of these two proteins can be observed.
Meanwhile, the expressions of both TNF-α and IFN-α increased
significantly, as determined by gene microarray analysis; however,
in the presence of TNF-α plus IFN-α inhibitors, TNF-α
and IFN-α did not increase in HeLa cells induced by coimmobilized
IFN-γ plus TNF-α. Our results indicate that such change
can stimilate HeLa cells to secrete more TNF-α and IFN-α,
by which the apoptosis of HeLa cells could be further induced. This
study is the first report of autocrine-induced apoptosis of HeLa cells.
In addition, we performed ELISA, RT-PCR, flow cytometry, and Western
blot analyses, as well as a series of analytical tests at the animal
level. our data also indicate that the PSt-coimmobilized IFN-γ
plus TNF-α has apparent effects for cancer treatment in vivo, which is of great significance for translation
into clinical medicine.