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Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics
journal contribution
posted on 2020-06-09, 00:43 authored by Yun Sun, Hong Liu, Yuanyuan Shen, Xingmei Huang, Fengge Song, Xiaolin Ge, Aimin Wang, Kaixiang Zhang, Yue Li, Chaoyang Li, Yi Wan, Jinghong LiField-deployable
detectors of disease biomarkers provide a simple
and fast analysis of clinical specimens. However, most of the existing
field-deployable diagnostics have poor sensitivity and are not suitable
for the detection of biomarkers with low abundance. Herein, we report
a highly sensitive and rapid colorimetric readout paper-based assay
for pathogen detection by integrating the unique collateral activity
of a Cas12a-activated universal field-deployable detector (CUFD).
The collateral effect of Cas12a results in a nonspecific destruction
of a fluorophore biotin-labeled ssDNA reporter for the CUFD. This
technique can quantify seven different kinds of pathogens in blood
samples without any purification procedure, with sensitivity as low
as 10 aM for the Shigella dysenteriae DNA. This CUFD technique has significant potential for the detection
of pathogenic DNA as well as other types of DNA or RNA targets at
the point-of-care application.
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Keywords
Bacterial Diagnostics Field-deployable detectorsCas 12a resultssensitivityblood samplescolorimetric readout paper-based assayfluorophore biotin-labeled ssDNA reporter10 aMfield-deployable detectordisease biomarkersCas 12acollateral effectShigella dysenteriae DNAcollateral activitypathogen detectionRNA targetsCUFD techniquefield-deployable diagnosticspurification procedureCas 12a Universal Field-Deployable Detectorspoint-of-care application
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