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Carbon Dioxide and Hydrogen Sulfide Associations with Regional Bacterial Diversity Patterns in Microbially Induced Concrete Corrosion
journal contribution
posted on 2014-07-01, 00:00 authored by Alison
L. Ling, Charles E. Robertson, J. Kirk Harris, Daniel N. Frank, Cassandra V. Kotter, Mark J. Stevens, Norman R. Pace, Mark T. HernandezThe microbial communities associated
with deteriorating concrete
corrosion fronts were characterized in 35 samples taken from wastewater
collection and treatment systems in ten utilities. Bacterial communities
were described using Illumina MiSeq sequencing of the V1V2 region
of the small subunit ribosomal ribonucleic acid (SSU-rRNA) gene recovered
from fresh corrosion products. Headspace gas concentrations (hydrogen
sulfide, carbon dioxide, and methane), pore water pH, moisture content,
and select mineralogy were tested for correlation to community outcomes
and corrosion extent using pairwise linear regressions and canonical
correspondence analysis. Corroding concrete was most commonly characterized
by moisture contents greater than 10%, pore water pH below one, and
limited richness (<10 taxa). Bacterial community composition was
not correlated to geographic location when considered independently
from other environmental factors. Corrosion was most severe in sites
with high levels of hydrogen sulfide (>100 ppm) and carbon dioxide
(>1%) gases, conditions which also were associated with low diversity
biofilms dominated by members of the acidophilic sulfur-oxidizer genus Acidithiobacillus.
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Keywords
treatment systemsV 1V regionBacterial communitiesmoisture contentmoisture contentsporecarbon DioxideHeadspace gas concentrationsDiversity Patternshydrogen sulfidepHcarbon dioxideIllumina MiSeq sequencingdiversity biofilmscorrosion frontscommunity outcomesMicrobially Induced Concrete CorrosionThecorrosion extentcorrosion productscanonical correspondence analysis35 sampleswastewater collectionHydrogen Sulfide Associations
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