jo060255g_si_001.pdf (989.03 kB)
Cap and Capture−Release Techniques Applied to Solid-Phase Synthesis of Oligosaccharides
journal contribution
posted on 2006-09-01, 00:00 authored by Jian Wu, Zhongwu GuoThis paper reports a new strategy for oligosaccharide
synthesis by combining solid-phase methods with cap and
capture−release separation techniques, using the p-(5-(ethoxycarbonyl)pentyloxy)benzyl group (CPB) as a tag for
the capture of desired oligosaccharides. After a complex
carbohydrate mixture was obtained by solid-phase synthesis,
the desired oligosaccharide containing a free carboxyl group
derived from CPB was attached to an amino resin. The
loaded resin was readily separated from side products by
filtration and finally treated with acid to release the pure
oligosaccharide product.