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Cancer Targeted Gene Therapy for Inhibition of Melanoma Lung Metastasis with eIF3i shRNA Loaded Liposomes
journal contribution
posted on 2019-12-10, 14:57 authored by Wen Xiao, Weiyi Zhang, Hai Huang, Yafei Xie, Yi Zhang, Xia Guo, Chaohui Jin, Xuelian Liao, Shaohua Yao, Guo Chen, Xiangrong SongEukaryotic translation initiation factors 3i (eIF3i)
is a proto-oncogene
that is overexpressed in various tumors, reducing its expression by
eIF3i shRNA is a promising strategy to inhibit tumor growth or metastasis.
Tumor cell is the target of eIF3i shRNA so that tumor-site accumulation
could be important for fulfilling its therapeutic effect. Thus, the
iRGD modified liposome (R-LP) was rationally synthesized to enhance
the antitumor effect by active targeted delivery of eIF3i shRNA to
B16F10 melanoma cells. R-LP encapsulating eIF3i shRNA gene (R-LP/sheIF3i)
were prepared by a film dispersion method. The transfection experiment
proves that R-LP could effectively transfect B16F10 cells. R-LP/sheIF3i
notably restrained the migration, invasion, and adhesion of melanoma
cells in vitro. In a mouse model of lung metastasis,
R-LP/sheIF3i administered by intravenous injection suppressed pulmonary
metastasis of melanoma by dramatically downregulated eIF3i expression
and subsequently inhibiting tumor neovascularization and tumor cells
proliferation in vivo. Our results provide a basis
for tumor cells targeting strategies to reduce the expression of eIF3i
by RNAi in the treatment of tumor metastasis.
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Keywords
Melanoma Lung MetastasisB 16F melanoma cellseIF 3i shRNAmetastasieIF 3i shRNA Loaded Liposomes Eukaryotic translation initiation factors 3 itransfect B 16F cellseIF 3icancer Targeted Gene TherapyR-LP encapsulating eIF 3i shRNA genedownregulated eIF 3i expressionfilm dispersion methodtumor cells proliferation
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