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Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering
journal contribution
posted on 2019-12-13, 20:43 authored by Mari Carmen Echave, Rui M. A. Domingues, Manuel Gómez-Florit, José Luis Pedraz, Rui L. Reis, Gorka Orive, Manuela E. GomesThe innate graded structural and compositional profile
of musculoskeletal
tissue interfaces is disrupted and replaced by fibrotic tissue in
the context of disease and degeneration. Tissue engineering strategies
focused on the restoration of the transitional complexity found in
those junctions present special relevance for regenerative medicine.
Herein, we developed a gelatin-based multiphasic hydrogel system,
where sections with distinct composition and microstructure were integrated
in a single unit. In each phase, hydroxyapatite particles or cellulose
nanocrystals (CNC) were incorporated into an enzymatically cross-linked
gelatin network to mimic bone or tendon tissue, respectively. Stiffer
hydrogels were produced with the incorporation of mineralized particles,
and magnetic alignment of CNC resulted in anisotropic structure formation.
The evaluation of the biological commitment with human adipose-derived
stem cells toward the tendon-to-bone interface revealed an aligned
cell growth and higher synthesis and deposition of tenascin in the
anisotropic phase, while the activity of the secreted alkaline phosphatase
and the expression of osteopontin were induced in the mineralized
phase. These results highlight the potential versatility offered by
gelatin-transglutaminase enzyme tandem for the development of strategies
that mimic the graded, composite, and complex intersections of the
connective tissues.
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Tissue engineering strategiesgelatin-transglutaminase enzyme tandemBiphasic Hydrogels Integrating Mineralizedmusculoskeletal tissue interfaceshydroxyapatite particlesanisotropic phaseregenerative medicineAnisotropic Featuresenzymatically cross-linked gelatin networktendon-to-bone interfacestrategyStiffer hydrogelsfibrotic tissuecell growthtendon tissueInterfacial Tissue EngineeringCNCanisotropic structure formationgelatin-based multiphasic hydrogel systemcellulose nanocrystals
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