cs0c00406_si_001.pdf (6.34 MB)
Biosynthesis of Cyclohexanecarboxyl-CoA Highlights a Promiscuous Shikimoyl-CoA Synthetase and a FAD-Dependent Dehydratase
journal contribution
posted on 2020-02-24, 14:34 authored by Will Skyrud, Antonio Del Rio Flores, Wenjun ZhangThe
conversion of shikimate to cyclohexanecarboxyl-CoA (CHC-CoA)
involves an exquisite orchestra of multiple enzymes to proceed stereospecifically
and avoid aromaticity, but little is known regarding the details of
this intriguing enzymatic reaction cascade. Through both in vitro
and in vivo analysis, we provide a plausible enzymatic pathway for
CHC-CoA biosynthesis from shikimate. This pathway highlights a shikimoyl-CoA
synthetase that is highly promiscuous toward a wide range of cycloalkane
and benzoate substrates. In addition, an acyl-CoA dehydrogenase is
shown to have evolved to be a FAD-dependent nonredox dehydratase that
is distinct from any known FAD-dependent dehydratases.
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CHC-CoA biosynthesisFAD-dependent dehydratasesBiosynthesiaromaticityconversionvivo analysisenzymeFAD-dependent nonredox dehydratasebenzoate substratespathwaycyclohexanecarboxyl-CoAshikimoyl-CoA synthetaseacyl-CoA dehydrogenasePromiscuous Shikimoyl-CoA Synthetasereaction cascadeCyclohexanecarboxyl-CoA HighlightscycloalkaneshikimatestereospecificallyFAD-Dependent Dehydratase
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