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Biomonitoring of Perfluorinated Compounds in a Drop of Blood
journal contribution
posted on 2015-12-17, 08:20 authored by Pan Mao, Daojing WangBiomonitoring
of pollutants and their metabolites and derivatives
using biofluids provides new opportunities for spatiotemporal assessment
of human risks to environmental exposures. Perfluorinated compounds
(PFCs) have been used widely in industry and pose significant environmental
concerns due to their stability and bioaccumulation in humans and
animals. However, current methods for extraction and measurement of
PFCs require relatively large volumes (over one hundred microliters)
of blood samples, and therefore, are not suitable for frequent blood
sampling and longitudinal biomonitoring of PFCs. We have developed
a new microassay, enabled by our silicon microfluidic chip platform,
for analyzing PFCs in small volumes (less than five microliters) of
blood. Our assay integrates on-chip solid-phase extraction (SPE) with
online nanoflow liquid chromatography-electrospray ionization-mass
spectrometry (nanoLC-ESI-MS) detection. We demonstrated high sample
recovery, excellent interday and intraday accuracy and precision,
and a limit of detection down to 50 femtogram of PFCs, in one microliter
of human plasma. We validated our assay performance using pooled human
plasma and NIST SRM 1950 samples. Our microfluidic chip-based assay
may enable frequent longitudinal biomonitoring of PFCs and other environmental
toxins using a finger prick of blood, thereby providing new insights
into their bioaccumulation, bioavailability, and toxicity.