nl5b03313_si_001.pdf (1.23 MB)
Biomimetic Self-Templated Hierarchical Structures of Collagen-Like Peptide Amphiphiles
journal contribution
posted on 2015-10-14, 00:00 authored by Hyo-Eon Jin, Jaein Jang, Jinhyo Chung, Hee Jung Lee, Eddie Wang, Seung-Wuk Lee, Woo-Jae ChungDeveloping hierarchically structured
biomaterials with tunable chemical and physical properties like those
found in nature is critically important to regenerative medicine and
studies on tissue morphogenesis. Despite advances in materials synthesis
and assembly processes, our ability to control hierarchical assembly
using fibrillar biomolecules remains limited. Here, we developed a
bioinspired approach to create collagen-like materials through directed
evolutionary screening and directed self-assembly. We first synthesized
peptide amphiphiles by coupling phage display-identified collagen-like
peptides to long-chain fatty acids. We then assembled the amphiphiles
into diverse, hierarchically organized, nanofibrous structures using
directed self-assembly based on liquid crystal flow and its controlled
deposition. The resulting structures sustained and directed the growth
of bone cells and hydroxyapatite biominerals. We believe these self-assembling
collagen-like amphiphiles could prove useful in the structural design
of tissue regenerating materials.