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Bioinspired Framework Nucleic Acid Capture Sensitively and Rapidly Resolving MicroRNAs Biomarkers in Living Cells
journal contribution
posted on 2020-02-25, 21:43 authored by Fan Yang, Yaru Cheng, Yiyi Zhang, Wei Wei, Haifeng Dong, Huiting Lu, Xueji ZhangQuantifying
intracellular microRNA (miRNA) is essential for diagnosis
and prognosis of diseases because of its importance to the development
and progression of complex diseases. The challenge is to develop methods
that enable multiplex miRNAs detection in ultralow amounts and over
broad concentration ranges. Inspired by the “tentacles”
of an octopus, herein, we present a framework nucleic acid (FNA) capture
for sensitive, rapid, and multiplexed imaging of miRNAs cancer biomarkers
in living cells. The programmable FNA is designed using three DNA
triangular prism (DTP) nanostructures carrying two pairs of metastable
catalytic hairpin assembled (CHA) probes, AS1411 aptamer, and pendent
biotinylated DNA strand in different vertexes and is further assembled
via streptavidin to form the multivalent DTP (SA-DTP). The SA-DTP
system acts as an octopus that captures the target cancer miRNAs quickly
and delivers them preferentially among DTPs’ “tentacles”
in the SA-DTP system to produce strong, amplified fluorescence for
detection. Precise multiplexed imaging of miRNA-155 and miRNA-21 cancer
biomarkers’ aberrant expression and dynamic change in different
cells demonstrates the feasibility of both monitoring disease progression
and evaluating therapeutic efficacy.
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ultralow amountsFNALiving Cells Quantifying intracellular microRNAPrecise multiplexed imagingCHABioinspired Framework NucleicCapture Sensitivelymultiplexed imagingmonitoring disease progressionSA-DTP system actstarget cancer miRNAsmiRNA -155SA-DTP systemmultiplex miRNAs detectionmiRNAs cancer biomarkersRapidly Resolving MicroRNAs Biomarkerspendent biotinylated DNA strandAS 1411 aptamerconcentration ranges
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