ab9b01493_si_001.pdf (564.43 kB)
Bioinspired, Artificial, Small-Diameter Vascular Grafts with Selective and Rapid Endothelialization Based on an Amniotic Membrane-Derived Hydrogel
journal contribution
posted on 2020-02-04, 13:47 authored by Xu Peng, Xu Wang, Can Cheng, Xiong Zhou, Zhipeng Gu, Li Li, Jun Liu, Xixun YuClinical application of the amniotic
membrane (AM) in vascular
reconstruction was limited by poor processability, rapid biodegradation,
and insufficient hemocompatibility. In this work, decellularized AM
was digested to a thermosensitive hydrogel and densely cross-linked
in the nanoscale as “enhanced” collagenous fibers. Via N-(3-dimehylaminopropyl)-N′-ethylcarbodiimide
and N-hydroxysuccinimide (EDC/NHS) catalysis, REDV
was further grafted to simulate anticoagulant substances on naturally
derived blood vessels. This modification approach endowed AM with
rapid endothelialization and rare vascular restenosis. Through adjusting
the fixation condition, the pore size and mechanical stability of
the fiber network were approximate to those of natural tissues and
precisely designed to fit for cell adhesion. AM was synchronously
fixed by alginate dialdehyde (ADA) and EDC/NHS, forming a “double-cross-linked”
stable structure with significantly improved mechanical strength and
resistance against enzymic degradation. The hemolytic and platelet
adhesion test indicated that ADA/REDV-AM could inhibit hemolysis and
coagulation. It also exhibited excellent cytocompatibility. It selectively
accelerated adsorption and migration of endothelial cells (ECs) while
impeding adhesion and proliferation of smooth muscle cells (SMCs).
It maintained EC superiority in competitive growth and avoided thrombosis
in vivo. Furthermore, its property of promoting reconstruction and
repair of blood vessels was proved in an animal experiment. Overall,
the present study demonstrates that ADA/REDV-AM has potential application
as a small-diameter artificial vascular intima with rapid endothelialization
and reduced SMC/platelet adhesion.