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Biofilm Impeding AgNPs Target Skin Carcinoma by Inducing Mitochondrial Membrane Depolarization Mediated through ROS Production
journal contribution
posted on 2016-10-07, 00:00 authored by Debasis Nayak, Manisha Kumari, Sripathi Rajachandar, Sarbani Ashe, Neethi Chandra Thathapudi, Bismita NayakReactive
oxygen species (ROS) are a double-edged sword that possesses both
beneficial and harmful effects. Although basic research on skin cancer
prevention has undergone a huge transformation, cases of recurrence
with higher rates of drug resistance are some of its drawbacks. Therefore,
targeting mitochondria by ROS overproduction provides an alternate
approach for anticancer therapy. In the present study, green-synthesized
silver nanoparticles (AgNPs) were explored for triggering the ROS
production in A431 skin carcinoma cells. The synthesized AgNPs were
characterized for size, charge, morphology, and phase through high-throughput
DLS, Fe-SEM, XRD, and ATR-FTIR techniques. Their physiochemical properties
with hemoglobin and blood plasma were screened through hemolysis,
hemagglutination assay, and circular dichroism spectroscopy confirmed
their nontoxic nature. The AgNPs also exhibited additional efficacy
in inhibiting biofilm produced by V. cholerae and B. subtilis, thereby facilitating
better applicability in wound-healing biomaterials. The depolarization
of mitochondrial membrane potential ΔΨm through excess
ROS production was deduced to be the triggering force behind the apoptotic
cell death mechanism of the skin carcinoma. Subsequent experimentation
through DNA fragmentation, comet tail formation, cell membrane blebbing,
and reduced invasiveness potentials through scratch assay confirmed
the physiological hallmarks of apoptosis. Thus, depolarizing mitochondrial
membrane potential through green-synthesized AgNPs provides an economic,
nontoxic, specific approach for targeting skin carcinoma with additional
benefits of antibacterial activities.
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skin cancer preventionΔΨROS productionassaycomet tail formationdepolarizing mitochondrial membraneInducing Mitochondrial Membrane Depolarization Mediatedapproachskin carcinomaXRDDLS431 skin carcinoma cellsROS Production Reactive oxygen speciesATR-FTIRapoptotic cell death mechanismDNAgreen-synthesized silver nanoparticlesBiofilm Impeding AgNPs Target Skin Carcinomacell membrane blebbing
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