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Biocatalytic Fabrication of α‑Glucan-Coated Porous Starch Granules by Amylolytic and Glucan-Synthesizing Enzymes as a Target-Specific Delivery Carrier
journal contribution
posted on 2019-10-11, 14:03 authored by Yi-Seul Jung, Moon-Gi Hong, Se-Hee Park, Byung-Hoo Lee, Sang-Ho YooIn this study, we
created biocatalytically
coated porous starch granules (PSGs) using amylosucrase from Neisseria polysaccharea to apply them as an encapsulant
for target-specific delivery. Field-emission scanning electron and
confocal laser scanning microscopic images showed that the PSGs were
completely concealed by the α-glucan coating layer. This carbohydrate-based
encapsulant displayed higher amount of resistant glucan contents due
to the elongated chains of the glucan coating, resulting in lower
digestibility of these PSGs in simulated digestive fluid systems.
Among the various PSGs evaluated, the highest loading efficiency for
the bioactive molecule crocin was observed with the β-amylase-induced
PSGs (β-PSGs) that had the smallest nanosize pores. Furthermore,
α-glucan-coated β-PSGs showed the highest capacity to
preserve the loaded crocin when incubated in simulated digestive fluids.
This suggests that the α-glucan-coated β-PSGs can potentially
be used for the delayed release of the core material in the upper
region of the gastrointestinal tract. Therefore, this system can be
potentially utilized as an effective carrier for colon-specific delivery,
and the release of the bioactive compound can be triggered by beneficial
intestinal microbiota.
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Keywords
colon-specific deliveryglucan coatingfluid systemscarbohydrate-based encapsulantloading efficiencyTarget-Specific Delivery CarrierNeisseria polysacchareacore materialα- glucan coating layerBiocatalytic FabricationGlucan-Synthesizing Enzymesglucan contentsα- glucan-coated β- PSGsnanosize poresbioactive molecule crocinstarch granulesbioactive compoundField-emission scanning electronconfocal laser scanningtarget-specific delivery
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