ml0c00094_si_001.pdf (668.11 kB)
Benzofuran-Based Carboxylic Acids as Carbonic Anhydrase Inhibitors and Antiproliferative Agents against Breast Cancer
Version 2 2020-03-23, 19:14
Version 1 2020-03-20, 13:51
journal contribution
posted on 2020-03-23, 19:14 authored by Wagdy M. Eldehna, Alessio Nocentini, Zainab M. Elsayed, Tarfah Al-Warhi, Nada Aljaeed, Ohoud J. Alotaibi, Mohammad M. Al-Sanea, Hatem A. Abdel-Aziz, Claudiu T. SupuranPursuing our effort
for developing effective inhibitors of the
cancer-related hCA IX isoform, here we describe the synthesis of novel
benzofuran-based carboxylic acid derivatives, featuring the benzoic
(9a–f) or hippuric (11a,b) acid moieties linked to 2-methylbenzofuran or 5-bromobenzofuran
tails via an ureido linker. The target carboxylic acids were evaluated
for the potential inhibitory action against hCAs I, II, IX, and XII.
Superiorly, benzofuran-containing carboxylic acid derivatives 9b, 9e, and 9f acted as submicromolar
hCA IX inhibitors with KIs = 0.91, 0.79, and 0.56 μM, respectively,
with selective inhibitory profile against the target hCA IX over the
off-target isoforms hCA I and II (SIs: 2 to >63
and
4–47, respectively). Compounds 9b, 9e, and 9f were examined for their antiproliferative action
against human breast cancer (MCF-7 and MDA-MB-231) cell lines. In
particular, 9e displayed promising antiproliferative
(IC50 = 2.52 ± 0.39 μM), cell cycle disturbance,
and pro-apoptotic actions in MDA-MB-231 cells.