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Bacterial Resistance Control on Mineral Surfaces of Hydroxyapatite and Human Teeth via Surface Charge-Driven Antifouling Coatings
journal contribution
posted on 2014-03-12, 00:00 authored by Antoine Venault, Hui-Shan Yang, Yen-Che Chiang, Bor-Shuinn Lee, Ruoh-Chyu Ruaan, Yung ChangThis
works reports a set of new functionalized polyethyleneimine (PEI)
polymers, including a neutral PEGylated polymer PEI-g-PEGMA, a negatively charged polymer PEI-g-SA, and
a zwitterionic polymer PEI-g-SBMA, and their use
as antibiofouling coating agent for human teeth protection. Polymers
were synthesized by Michael addition, XPS analysis revealed that each
polymer could be efficiently coated onto hydroxyapatite, ceramic material
used as a model tooth. Polymers carrying a negative net charge were
more efficiently adsorbed, because of the establishment of electrostatic
interactions with calcium ions. Protein adsorption tests revealed
that two factors were important in the reduction of protein adsorption.
Both the surface charge and the surface ability to bind and entrap
water molecules had to be considered. PEI-g-SBMA,
which zeta potential in PBS solution was negative, was efficient to
inhibit the adsorption of BSA, a negative protein. On the other hand,
it also resisted the adsorption of lysozyme, a positive protein, because
zwitterionic molecules can easily entrap water and provide a very
hydrophilic environment. Streptococcus mutans attachment
tests performed unveiled that all modified polymers were efficient
to resist this type of bacteria responsible for dental carries. Best
results were also obtained with PEI-g-SBMA coating.
This polymer was also shown to efficiently resist the adsorption of
positively charged bacteria (Stenotrophomonas maltophilia). Tests performed on real human tooth showed that PEI-g-SBMA could inhibit up to 70% of bacteria adhesion, which constitutes
a major result considering that surface of teeth is very rough, therefore
physically promoting the attachment of proteins and bacteria.
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Keywords
functionalized polyethyleneiminebacteria adhesionBacterial Resistance Controlantibiofouling coating agentProtein adsorption testscalcium ionsentrap watersurface abilityHuman Teethsurface chargeStreptococcus mutans attachment testsentrap water moleculesMineral SurfacesPEIMichael additionzwitterionic moleculesStenotrophomonas maltophiliaPBS solutionmodel toothBSApolymerXPS analysisteeth protectionprotein adsorption
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