nl5b04458_si_002.avi (6.86 MB)
Axon-First Neuritogenesis on Vertical Nanowires
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posted on 2016-01-13, 00:00 authored by Kyungtae Kang, Yi-Seul Park, Matthew Park, Min Jee Jang, Seong-Min Kim, Juno Lee, Ji Yu Choi, Da Hee Jung, Young-Tae Chang, Myung-Han Yoon, Jin Seok Lee, Yoonkey Nam, Insung S. ChoiIn this work, we report that high-density,
vertically grown silicon nanowires (vg-SiNWs) direct
a new in vitro developmental pathway of primary hippocampal
neurons. Neurons on vg-SiNWs formed a single, extremely
elongated major neurite earlier than minor neurites, which led to
accelerated polarization. Additionally, the development of lamellipodia,
which generally occurs on 2D culture coverslips, was absent on vg-SiNWs. The results indicate that surface topography is
an important factor that influences neuronal development and also
provide implications for the role of topography in neuronal development in vivo.