nl1026056_si_001.pdf (1.39 MB)
Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
journal contribution
posted on 2015-12-16, 19:25 authored by Richard S. Gaster, Drew A. Hall, Shan X. WangWe report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.