jf8b04724_si_001.pdf (1.56 MB)
Assessment of Antinutritional Compounds and Chemotaxonomic Relationships between Camelina sativa and Its Wild Relatives
journal contribution
posted on 2018-12-20, 00:00 authored by Lisa Amyot, Tim McDowell, Sara L. Martin, Justin Renaud, Margaret Y. Gruber, Abdelali HannoufaWe compared the secondary metabolite
composition in seeds of Camelina sativa and its wild
relatives to identify potential
germplasm with reduced levels of antinutritional compounds. Twenty
Camelina accessions, from five different species, were analyzed by
liquid chromatography mass spectrometry and subjected to principal
component analysis, which revealed that Camelina spp. separated into distinct chemotaxonomic groups. Three
major glucosinolates (GSs) were identified in our study, namely, 9-methylsulfinylnonyl
GS (GS9), 10-methylsulfinyldecyl GS (GS10), and 11-methylsulfinylundecyl GS
(GS11). While there were differences in total GS levels, species-specific
patterns for GS9 and GS11 were noted. Sinapine content ranged between
1.4 and 5.6 mg/g FW, with the lowest levels observed in C. laxa and C. sativa. Lignin levels were
also lowest in C. sativa, with most accessions containing
less than 6 mg/g FW. Our results show that wild Camelina spp. have distinct metabolomes, and based on their
levels of major antinutritionals, some could be incorporated into
breeding programs with C. sativa.