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Artepillin C, a Key Component of Brazilian Propolis, Induces Thermogenesis in Inguinal White Adipose Tissue of Mice through a Creatine-Metabolism-Related Thermogenic Pathway
journal contribution
posted on 2020-01-18, 20:13 authored by Sho Nishikawa, Takuma Hydo, Hiroki Aoyama, Ryo Miyata, Shigenori Kumazawa, Takanori TsudaInduction
of beige adipocytes in white adipose tissue (WAT) is
a potential therapeutic target for the treatment of obesity because
beige adipocytes release excess energy via uncoupling-protein-1-associated
thermogenesis. In this study, we investigated how artepillin C (ArtC)
promotes thermogenesis in vivo. We demonstrated that
28 day administration of ArtC (10 mg/kg of body weight) to mice significantly
induced thermogenesis in beige adipocytes in inguinal WAT (iWAT) and
suppressed reductions in core body temperature induced by cold exposure
at 4 °C. Moreover, ArtC-induced thermogenesis in iWAT was significantly
inhibited by treatment with a creatine metabolism inhibitor, and ArtC
significantly upregulated the expression of creatine-metabolism-related
enzymes in the thermogenic pathway. These results indicate that ArtC
induces thermogenesis in beige adipocytes in iWAT, and the observed
ArtC-induced thermogenesis is associated with the creatine-metabolism-related
thermogenic pathway, which is characteristically observed in beige
adipocytes.