Arg-Glu-Asp-Val Peptide Immobilized on an Acellular
Graft Surface Inhibits Platelet Adhesion and Fibrin Clot Deposition
in a Peptide Density-Dependent Manner
Acellular
blood vessels possess high potential to be used as tissue-engineered
vascular scaffolds. Previously, a high patency was achieved for an
Arg-Glu-Asp-Val (REDV) peptide-immobilized small-diameter acellular
graft in a minipig model. Results revealed the potential of the peptide
to capture a circulating cell and also to suppress fibrin clot deposition.
Here, the effect of REDV peptide density on the blood response under ex vivo blood perfusion conditions was investigated. When
endothelial cells or platelets were seeded under static conditions,
the number of adherent endothelial cells increased with the increase
in peptide density. Platelets scarcely adhered on the surface where
the peptide density was above 18.9 × 10–4 molecules
per nm3. Fibrin clot deposition and circulating cell capture
were evaluated in a minipig extracorporeal circulatory system. The
fibrin clot did not form on the peptide-immobilized surface, in the
range of peptide modification density that was evaluated, whereas
the unmodified surface was covered with microthrombi. REDV-specific
blood circulating cells were captured on the peptide-immobilized surface
with a density above 18.9 × 10–4 molecules
per nm3. These results illustrated, under ex vivo blood perfusion conditions, that the REDV-immobilized acellular
surface was able to capture cells and also suppress platelet adhesion
and fibrin clot deposition in a peptide density-dependent manner.