bm9b01060_si_001.pdf (1.12 MB)
Antimicrobial Peptide-Conjugated Hierarchical Antifouling Polymer Brushes for Functionalized Catheter Surfaces
journal contribution
posted on 2019-10-17, 17:35 authored by Xin-Yang Zhang, Yu-Qing Zhao, Yidan Zhang, Anzhi Wang, Xiaokang Ding, Yang Li, Shun Duan, Xuejia Ding, Fu-Jian XuCatheter-related
infection is a great challenge to modern medicine,
which causes significant economic burden and increases patient morbidity.
Hence, there is a great requirement for functionalized surfaces with
inherently antibacterial properties and biocompatibility that prevent
bacterial colonization and attachment of blood cells. Herein, we developed
a strategy for constructing polymer brushes with hierarchical architecture
on polyurethane (PU) via surface-initiated atom-transfer radical polymerization
(SI-ATRP). Surface-functionalized PU (PU-DMH) was readily prepared,
which comprised of poly(3-[dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azaniumyl]propane-1-sulfonate)
(PDMAPS) brushes as the lower layer and antimicrobial peptide-conjugated
poly(methacrylic acid) (PMAA) brushes as the upper layer. The PU-DMH
surface showed excellent bactericidal property against both Gram-positive
and Gram-negative bacteria and could prevent accumulation of bacterial
debris on surfaces. Simultaneously, the PU-DMH samples possessed good
hemocompatibility and low cytotoxicity. Furthermore, the integrated
antifouling and bactericidal properties of PU-DMH under hydrodynamic
conditions were confirmed by an in vitro circulating model. The functionalized
surface possessed persistent antifouling and bactericidal performances
both under static and hydrodynamic conditions. The microbiological
and histological results of animal experiments also verified the in
vivo anti-infection performance. The present work might find promising
clinical applications for preventing catheter-related infection.