id9b00379_si_002.mpg (3.96 MB)
Antibacterial Photodynamic Inactivation of Antibiotic-Resistant Bacteria and Biofilms with Nanomolar Photosensitizer Concentrations
media
posted on 2020-01-16, 19:41 authored by Carolina S. Vinagreiro, Amanda Zangirolami, Fabio A. Schaberle, Sandra C. C. Nunes, Kate C. Blanco, Natalia M. Inada, Gabriela Jorge da Silva, Alberto A. C. C. Pais, Vanderlei S. Bagnato, Luis G. Arnaut, Mariette M. PereiraGram-negative
bacteria and bacteria in biofilms are very difficult to eradicate
and are the most antibiotic-resistant bacteria. Therapeutic alternatives
less susceptible to mechanisms of resistance are urgently needed to
respond to an alarming increase of resistant nosocomial infections.
Antibacterial photodynamic inactivation (PDI) generates oxidative
stress that triggers multiple cell death mechanisms that are more
difficult to counteract by bacteria. We explore PDI of multidrug-resistant
bacterial strains collected from patients and show how positive charge
distribution in the photosensitizer drug impacts the efficacy of inactivation.
We demonstrate the relevance of size for drug diffusion in biofilms.
The designed meso-imidazolyl porphyrins of small
size with positive charges surrounding the macrocycle enabled the
inactivation of bacteria in biofilms by 6.9 log units at 5 nM photosensitizer
concentration and 5 J cm–2, which offers new opportunities
to treat biofilm infections.
History
Usage metrics
Keywords
6.9 log unitsNanomolar Photosensitizer Concentrationsdrug diffusioncell death mechanisms5 nM photosensitizer concentrationantibiotic-resistant bacteriacharge distributionphotosensitizer drug impactsAntibacterial Photodynamic Inactivationnosocomial infectionsimidazolyl porphyrinsAntibiotic-Resistant BacteriaAntibacterial photodynamic inactivationPDITherapeutic alternativesoxidative stressbiofilm infections
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC