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Anti-CD133 Antibody-Targeted Therapeutic Immunomagnetic Albumin Microbeads Loaded with Vincristine-Assisted to Enhance Anti-Glioblastoma Treatment
journal contribution
posted on 2019-10-15, 14:37 authored by Xueqin Wang, Ping Lu, Li Zhu, Li Qin, Yongxia Zhu, Guoyi Yan, Shaofeng Duan, Yuqi GuoPoor uptake of antitumor drugs by
tumor cells is a critical challenge
for anticancer therapeutics. Moreover, the deficiency of specific
tumor selectivity for tumor sites may further limit the therapeutic
efficacy and cause side effects in healthy regions of the body. Vincristine
(VCR) is an effective antitumor drug; however, because of its severe
nerve toxicity, short half-life, and fast metabolism, its clinical
application is limited. Herein, novel anti-CD133 monoclonal antibody
(CD133mAb)-targeted therapeutic immunomagnetic albumin
microbeads (CD133mAb/TMAMbs) are smartly constructed for
enhancing antiglioblastoma treatment. Superparamagnetic iron oxide
nanoparticles (SPIO NPs) were first fabricated as nanocarrier cores,
then encapsulated with human serum albumin (HSA), and loaded antitumor
drug VCR. Then CD133mAb, which has specific affinity with
the cell membrane CD133, was subsequently conjugated to form CD133mAb-decorated therapeutic immunomagnetic albumin microbeads
(CD133mAb/TMAMbs). The influence of CD133mAb/TMAMbs
on the viability, cell cycle, apoptosis, cell cytoskeleton, migration,
and invasion of CD133-overexpressing U251 cells was explored. The CD133mAb-conjugated magnetic albumin microbeads exhibited a
high drug loading capacity, stability and hemocompatibility, and active
targeting ability by specific recognition of the CD133 surface antigen
by the bioconjugation of CD133mAb. More importantly, the
constructed therapeutic CD133mAb/TMAMbs have a specifically
effective uptake via the CD133 transmembrane protein that is overexpressed
in U251 glioblastoma cells and displayed an effective antitumor proliferation
and invasive ability. Therefore, based on these results, the fabricated CD133mAb/TMAMbs demonstrate promising uses in brain cancer-targeted
diagnosis and therapy.
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CD 133 transmembrane proteinEnhance Anti-Glioblastoma Treatment Poor uptakeHSAU 251 glioblastoma cellsSPIO NPsantiglioblastoma treatmenttumor sitesnovel anti-CD 133CD 133 mAb-conjugatedantitumor drugsdrug loading capacitybrain cancer-targeted diagnosistumor cellscell cytoskeletonantitumor drug VCRcause side effectsimmunomagnetic albumin microbeadsantitumor drugCD 133 mAbtumor selectivityalbumin microbeadsantitumor proliferationCD 133 surface antigenCD 133-overexpressing U 251 cellsAnti-CD 133 Antibody-Targeted Therapeutic Immunomagnetic Albumin Microbeads LoadedSuperparamagnetic iron oxide nanoparticlesanticancer therapeuticscell cyclenerve toxicitynanocarrier coresform CD 133 mAb-decoratedserum albumin
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