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An Electrochemical Biosensor Designed by Using Zr-Based Metal–Organic Frameworks for the Detection of Glioblastoma-Derived Exosomes with Practical Application
journal contribution
posted on 2020-02-14, 22:03 authored by Zhaowei Sun, Lei Wang, Shuai Wu, Yanhong Pan, Yu Dong, Sha Zhu, Jie Yang, Yongmei Yin, Genxi LiGlioblastoma
(GBM) is one of the most fatal tumors in the brain,
and its early diagnosis remains technically challenging due to the
complex repertoires of oncogenic alterations and blood-brain barrier
(BBB). GBM-derived specific exosomes can cross the BBB and circulate
in body fluids, so they can be noninvasive biomarkers for the early
diagnosis of GBM. Herein, we propose a sensitive and label-free electrochemical
biosensor designed by using Zr-based metal–organic frameworks
(Zr-MOFs) for the detection of GBM-derived exosomes with practical
application. In the design, a peptide ligand can specifically bind
with human epidermal growth factor receptor (EGFR) and EGFR variant
(v) III mutation (EGFRvIII), which are overexpressed on the GBM-derived
exosomes. Meanwhile, Zr-MOFs encapsulated with methylene blue can
absorb on the surface of the exosomes due to the interaction between
Zr4+ and the intrinsic phosphate groups outside of exosomes.
Consequently, the concentration of exosomes can be directly quantified
by monitoring the electroactive molecules inside MOFs, ranging from
9.5 × 103 to 1.9 × 107 particles/μL
with the detection of limit of 7.83 × 103 particles/μL.
Furthermore, this proposed biosensor can distinguish GBM patients
from healthy groups, demonstrating the great prospect for early clinical
diagnosis.